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Clinical Biochemistry: Spectrophotometry is the gold standard for enzyme assays (e.g., measuring liver enzyme activity) and determining metabolite concentrations like glucose or hemoglobin in blood samples.
Molecular Biology: It is essential for DNA/RNA quantification. The absorbance ratio at 260nm and 280nm indicates nucleic acid purity relative to protein contamination.
Environmental Science: The technique is used to monitor water quality by detecting heavy metals and organic pollutants through colorimetric reactions.

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Prioritized list of optimization measures, including cost-benefit analysis and payback period estimates.

4. Applications

Clinical Biochemistry: Spectrophotometry is the gold standard for enzyme assays (e.g., measuring liver enzyme activity) and determining metabolite concentrations like glucose or hemoglobin in blood samples.
Molecular Biology: It is essential for DNA/RNA quantification. The absorbance ratio at 260nm and 280nm indicates nucleic acid purity relative to protein contamination.
Environmental Science: The technique is used to monitor water quality by detecting heavy metals and organic pollutants through colorimetric reactions.